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recombinant type xxiii collagen ectodomain  (R&D Systems)


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    R&D Systems recombinant type xxiii collagen ectodomain
    Recombinant Type Xxiii Collagen Ectodomain, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+collagen+xxiii/Recombinant+Human+Collagen+XXIII+alpha+1%2FCOL23A1+Protein%2C+CF/us12391747-215-3-9
    Average 93 stars, based on 1 article reviews
    recombinant type xxiii collagen ectodomain - by Bioz Stars, 2026-10
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    Biomarker Discovery:

    Article Title: Collagen XXIII--a potential biomarker for the detection of primary and recurrent non-small cell lung cancer
    Article Snippet: Antibody Validation A new human collagen XXIII mouse monoclonal antibody was made against the cleaved portion of human collagen XXIII by R&D Systems. .. Antibody Validation A new human collagen XXIII mouse monoclonal antibody was made against the cleaved portion of human collagen XXIII by R&D Systems. .. 20 μg of whole-cell lysates from H460 cells expressing pRS or pRS-shc23 were resolved on a 7.5% Tris-HCl gel and transferred to a 0.45μM PVDF membrane (Millipore).



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    Specificity assessment of the PRO‐C23 antibody <t>10F6.</t> (a) Sequence alignment for C‐terminus of type XIII, XXIII and XXV collagens. The antibody recognises the residues from 531 to 540 of type 23 collagen. (b) Western blot results of recombinant type 23 collagen using 10F6 as a primary antibody. (c) PRO‐C23 antibody specificity towards different peptides. Reactivity to the type XXIII collagen selection peptide (GLPVPGCWHK), the elongated peptide (GLPVPGCWHKA), the truncated peptide (GLPVPGCWH), a peptide from type XIII collagen (GLPVQGCWNK) and peptide from type XXV collagen (GLPMPGCWQK) was tested in the PRO‐C23 assay. (d) Correlations of PRO‐C23 levels serum levels with the levels of the EDTA, heparin and citrate plasma ( n = 16). PRO‐C23: biomarker of the ectodomain of type 23 collagen; OD: optical density; EDTA: ethylenediaminetetraacetic acid
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    R&D Systems type 23 collagen antibody ab
    Specificity assessment of the PRO‐C23 antibody <t>10F6.</t> (a) Sequence alignment for C‐terminus of type XIII, XXIII and XXV collagens. The antibody recognises the residues from 531 to 540 of type 23 collagen. (b) Western blot results of recombinant type 23 collagen using 10F6 as a primary antibody. (c) PRO‐C23 antibody specificity towards different peptides. Reactivity to the type XXIII collagen selection peptide (GLPVPGCWHK), the elongated peptide (GLPVPGCWHKA), the truncated peptide (GLPVPGCWH), a peptide from type XIII collagen (GLPVQGCWNK) and peptide from type XXV collagen (GLPMPGCWQK) was tested in the PRO‐C23 assay. (d) Correlations of PRO‐C23 levels serum levels with the levels of the EDTA, heparin and citrate plasma ( n = 16). PRO‐C23: biomarker of the ectodomain of type 23 collagen; OD: optical density; EDTA: ethylenediaminetetraacetic acid
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    Specificity assessment of the PRO‐C23 antibody 10F6. (a) Sequence alignment for C‐terminus of type XIII, XXIII and XXV collagens. The antibody recognises the residues from 531 to 540 of type 23 collagen. (b) Western blot results of recombinant type 23 collagen using 10F6 as a primary antibody. (c) PRO‐C23 antibody specificity towards different peptides. Reactivity to the type XXIII collagen selection peptide (GLPVPGCWHK), the elongated peptide (GLPVPGCWHKA), the truncated peptide (GLPVPGCWH), a peptide from type XIII collagen (GLPVQGCWNK) and peptide from type XXV collagen (GLPMPGCWQK) was tested in the PRO‐C23 assay. (d) Correlations of PRO‐C23 levels serum levels with the levels of the EDTA, heparin and citrate plasma ( n = 16). PRO‐C23: biomarker of the ectodomain of type 23 collagen; OD: optical density; EDTA: ethylenediaminetetraacetic acid

    Journal: United European Gastroenterology Journal

    Article Title: Elevated ectodomain of type 23 collagen is a novel biomarker of the intestinal epithelium to monitor disease activity in ulcerative colitis and Crohn's disease

    doi: 10.1177/2050640620977371

    Figure Lengend Snippet: Specificity assessment of the PRO‐C23 antibody 10F6. (a) Sequence alignment for C‐terminus of type XIII, XXIII and XXV collagens. The antibody recognises the residues from 531 to 540 of type 23 collagen. (b) Western blot results of recombinant type 23 collagen using 10F6 as a primary antibody. (c) PRO‐C23 antibody specificity towards different peptides. Reactivity to the type XXIII collagen selection peptide (GLPVPGCWHK), the elongated peptide (GLPVPGCWHKA), the truncated peptide (GLPVPGCWH), a peptide from type XIII collagen (GLPVQGCWNK) and peptide from type XXV collagen (GLPMPGCWQK) was tested in the PRO‐C23 assay. (d) Correlations of PRO‐C23 levels serum levels with the levels of the EDTA, heparin and citrate plasma ( n = 16). PRO‐C23: biomarker of the ectodomain of type 23 collagen; OD: optical density; EDTA: ethylenediaminetetraacetic acid

    Article Snippet: Western blot of recombinant type 23 collagen ectodomain (4165‐CL, R&D system) showed that the chosen antibody 10F6 recognised type 23 collagen ectodomain around 60 kD, while the reference commercial antibody (MAB4165, R&D system) was also shown (Figure ).

    Techniques: Sequencing, Western Blot, Recombinant, Selection, Clinical Proteomics, Biomarker Discovery